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human dnmt3b1 cdna  (Addgene inc)


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    Structured Review

    Addgene inc human dnmt3b1 cdna
    Human Dnmt3b1 Cdna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+dnmt3b1+cdna/pcdna3+1/pm38291337-497-10-15
    Average 90 stars, based on 1 article reviews
    human dnmt3b1 cdna - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Protein Purification:

    Article Title: DNMT3B PWWP mutations cause hypermethylation of heterochromatin.
    Article Snippet: .. Protein purification For recombinant expression DNMT3B fragments were cloned using human DNMT3B1 cDNA as template (Addgene #35552) performing ligation-independent cloning into 6xHis-Maltose binding protein (MBP) plasmid (Addgene plasmid # 29656). .. Mutants were incorporated using QuikChange II site-directed mutagenesis kit (Agilent) or direct cloning of synthesised doublestrand gBlock fragments containing mutations (Integrated DNA technologies).

    Recombinant:

    Article Title: DNMT3B PWWP mutations cause hypermethylation of heterochromatin.
    Article Snippet: .. Protein purification For recombinant expression DNMT3B fragments were cloned using human DNMT3B1 cDNA as template (Addgene #35552) performing ligation-independent cloning into 6xHis-Maltose binding protein (MBP) plasmid (Addgene plasmid # 29656). .. Mutants were incorporated using QuikChange II site-directed mutagenesis kit (Agilent) or direct cloning of synthesised doublestrand gBlock fragments containing mutations (Integrated DNA technologies).

    Expressing:

    Article Title: DNMT3B PWWP mutations cause hypermethylation of heterochromatin.
    Article Snippet: .. Protein purification For recombinant expression DNMT3B fragments were cloned using human DNMT3B1 cDNA as template (Addgene #35552) performing ligation-independent cloning into 6xHis-Maltose binding protein (MBP) plasmid (Addgene plasmid # 29656). .. Mutants were incorporated using QuikChange II site-directed mutagenesis kit (Agilent) or direct cloning of synthesised doublestrand gBlock fragments containing mutations (Integrated DNA technologies).

    Clone Assay:

    Article Title: DNMT3B PWWP mutations cause hypermethylation of heterochromatin.
    Article Snippet: .. Protein purification For recombinant expression DNMT3B fragments were cloned using human DNMT3B1 cDNA as template (Addgene #35552) performing ligation-independent cloning into 6xHis-Maltose binding protein (MBP) plasmid (Addgene plasmid # 29656). .. Mutants were incorporated using QuikChange II site-directed mutagenesis kit (Agilent) or direct cloning of synthesised doublestrand gBlock fragments containing mutations (Integrated DNA technologies).

    Ligation:

    Article Title: DNMT3B PWWP mutations cause hypermethylation of heterochromatin.
    Article Snippet: .. Protein purification For recombinant expression DNMT3B fragments were cloned using human DNMT3B1 cDNA as template (Addgene #35552) performing ligation-independent cloning into 6xHis-Maltose binding protein (MBP) plasmid (Addgene plasmid # 29656). .. Mutants were incorporated using QuikChange II site-directed mutagenesis kit (Agilent) or direct cloning of synthesised doublestrand gBlock fragments containing mutations (Integrated DNA technologies).

    Cloning:

    Article Title: DNMT3B PWWP mutations cause hypermethylation of heterochromatin.
    Article Snippet: .. Protein purification For recombinant expression DNMT3B fragments were cloned using human DNMT3B1 cDNA as template (Addgene #35552) performing ligation-independent cloning into 6xHis-Maltose binding protein (MBP) plasmid (Addgene plasmid # 29656). .. Mutants were incorporated using QuikChange II site-directed mutagenesis kit (Agilent) or direct cloning of synthesised doublestrand gBlock fragments containing mutations (Integrated DNA technologies).

    Binding Assay:

    Article Title: DNMT3B PWWP mutations cause hypermethylation of heterochromatin.
    Article Snippet: .. Protein purification For recombinant expression DNMT3B fragments were cloned using human DNMT3B1 cDNA as template (Addgene #35552) performing ligation-independent cloning into 6xHis-Maltose binding protein (MBP) plasmid (Addgene plasmid # 29656). .. Mutants were incorporated using QuikChange II site-directed mutagenesis kit (Agilent) or direct cloning of synthesised doublestrand gBlock fragments containing mutations (Integrated DNA technologies).

    Plasmid Preparation:

    Article Title: DNMT3B PWWP mutations cause hypermethylation of heterochromatin.
    Article Snippet: .. Protein purification For recombinant expression DNMT3B fragments were cloned using human DNMT3B1 cDNA as template (Addgene #35552) performing ligation-independent cloning into 6xHis-Maltose binding protein (MBP) plasmid (Addgene plasmid # 29656). .. Mutants were incorporated using QuikChange II site-directed mutagenesis kit (Agilent) or direct cloning of synthesised doublestrand gBlock fragments containing mutations (Integrated DNA technologies).



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    human dnmt3b1 cdna  (Addgene inc)
    90
    Addgene inc human dnmt3b1 cdna
    Human Dnmt3b1 Cdna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+dnmt3b1+cdna/pcdna3+1/pm38291337-497-10-15
    Average 90 stars, based on 1 article reviews
    human dnmt3b1 cdna - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

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